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Once they had the sequences, they synthesised the most elastic fragment of the proteins in the laboratory, and using an atomic force microscope available at nano GUNE, they were able to measure the mechanical resistance of each of the proteins.

"This instrument allows us, literally, to take a protein and stretch it, unfold it mechanically using force, which is something similar to what happens to titin in the muscle," said the researcher.

For titin this is a particularly appropriate approach given its function," said Pérez-Jiménez.

For this research, they selected over thirty animals from different taxonomic groups and of different sizes.

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"The complete genome of many animals was already available, so the first thing we did was to build a phylogenetic tree with the titin sequences of around thirty tetrapods.

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